Radiotherapy/Experimental Runs/2020-02-04: Difference between revisions

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Initial measurements with Cyberknife at Birmingham.
Second experimental measurements with Cyberknife at Birmingham.
Blue scintillator thin sheet measurements only.
Green scintillator thin sheet measurements only.


== Experiment Equipment ==
== Setup ==
{| class="wikitable"
 
! style="text-align: center;" | Item
=== Schematic ===
! style="text-align: center;" | Notes
|-
| style="text-align: center;" | Network Hub
| style="text-align: center;" | Set in control room to take output from experimental room ethernet connection. Control laptops connected via 5GHz WiFi.
|-
| style="text-align: center;" | Control Laptop x3
| style="text-align: center;" | 2 for remote control of CMOS sensor and FPGA, 1 for notes/web GUI.
|-
| style="text-align: center;" | Ethernet Cable x 3
| style="text-align: center;" | To connect DAQ laptops to network in the experimental room, network hub to network in control room.
|-
| style="text-align: center;" | Portable Enclosure
| style="text-align: center;" | Modified Big Zarges Waterproof Wheeled Equipment Case.
Features mount for scintillator stack, front and back openings for beam, patch panel with ports for SHV, BNC, SMA, USB, Camera Link cables. <br/>
Hole for sensor powering. <br/>
Mylar windows with Al support mounted to internal face: approximately light-tight.
|-
| style="text-align: center;" | Rev. B Scintillator stack
| style="text-align: center;" | 49 (25 clear, 23 white painted and 1 black painted) X x 100 mm, Y x 100 mm and Z x (approx) 2.8 mm sheets in ascending order from the front/beam of the end of the scintillator. Sheet numbers from front to end (in beam direction gantry angle 270°): 79, 68, 73, 62, 81, 72, 64, 67, 65, 71, 78, 77, 74, 75, 85, 82, 83, 76, 70, 80, 69, 84, 63, 66, 61, 34, 43, 32, 8, 40, 37, 38, 47, 46, 33, 42, 9, 39, 44, 49, 50, 31, 1, 45, 3, 54, 5, 51, 26. Full stack thickness measurements (in mm): 138.12, 137.74, 137.74.
|-
| style="text-align: center;" | Rev. C Scintillator stack
| style="text-align: center;" | 32 X x 105 mm, Y x 105 mm and Z x (approx) 3 mm sheets (21 3.4 mm and 11 2.0 mm) in ascending order from the front/beam of the end of the scintillator. Sheet numbers from front to end (in beam direction gantry angle 90°): 117, 113, 134, 110, 108, 128, 107, 106, 127, 105, 104, 125, 103, 102, 124, 101, 98, 123, 97, 96, 122, 94, 93, 121, 92, 90, 120, 89, 88, 119, 87, 135. Full stack thickness measurements (in mm): 95.87, 95.96, 95.97.
|-
| style="text-align: center;" | DAQ laptop x2
| style="text-align: center;" | Control CMOS sensor and photodiode acquisition.
|-
| style="text-align: center;" | ISDI CMOS sensor
| style="text-align: center;" | sensor pixel dimension: 1030 x 1536. NO optical grease between scintillator and sensor. Connected to DAQ PC via Camera Link cable.
|-
| style="text-align: center;" | Nexys Video FPGA development board.
| style="text-align: center;" | For interfacing between DDC232 and PC.
|-
| style="text-align: center;" | Rev. B Texas Instruments DDC232 custom circuit board (x3)
| style="text-align: center;" | Each housing 16x Hamamatsu S12915-16R photodiodes, coupled directly to scintillator sheets. Aligned by eye to scintillator sheets.
|-
| style="text-align: center;" | Rev. C Texas Instruments DDC232 custom circuit board (x1)
| style="text-align: center;" | Housing 32x Hamamatsu S12915-16R photodiodes, coupled directly to scintillator sheets. Aligned by eye to scintillator sheets.
|-
| style="text-align: center;" | Gloves
| style="text-align: center;" | For handling scintillator
|}
==== Schematic ====
<div class="image600px" style="text-align: center;">
<div class="image600px" style="text-align: center;">
[http://www.hep.ucl.ac.uk/pbt/wikiData/images/UCLH_Nov2021/UCL_Setup.png http://www.hep.ucl.ac.uk/pbt/wikiData/images/UCLH_Jun2022/UCL_Setup.png]
https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/setup.png
</div>
</div>


== Experiment Plan ==
=== Pre-data Taking Information and Notes ===


'''Rev. B Prototype'''
* Phantom
*Background & calibration shoot-throughs (background at all 8 DDC232 full-scale ranges – likely 12.5 pC is most appropriate FSR)
** 200mmx200mmx2mm blue scintillator sheet polystyrene base
*Pencil beams, 70-130 MeV, 10 MeV steps, in both directions into stack. For direction with Bragg peak in clear sheets, test 2 currents (high/low)
** sandwiched by two 200mmx200mmx50mm polystyrene clear plastic blocks
* 565mm from lens to sheet
* Check camera in focus with ruler on the sheet
* Ruler in focus at sheet
* Scintillator blocks aligned to laser cross hairs
* 785 mm source to surface distance suitable for output factor/PDD/profile measurements.
* Wooden box is covered with black cloth
* Camera height adjusted with wooden plates
* Top surfaces of plastic blocks and sheet aligned with paper underneath


'''Rev. C Prototype'''
== Data Taking ==
*Background & calibration shoot-throughs (12.5 pC FSR)
* All images taken with
*Pencil beams, 70-110 MeV, 5 MeV steps, at clinical current.
** Canon EOS 1100D
** 18-55mm lens
** f/5
** 200ISO
** 30s exposure
* Beam turned on for a given amount of time within the 30s exposure
* This is noted in monitor units, with the beam having constant 1000MU/minute


== Experiment Notes ==
* Used EOS utility app for controlling camera
* Image of ruler taken at sheet for pixels to mm conversion
* 3 images taken at each collimator size with 1 background image each.


Range calorimeter measurements with CMOS sensor + photodiodes.
<!-- ------------------------------------table3------------------------------------------ -->


Images from Sensor: '''[http://www.hep.ucl.ac.uk/pbt/wikiData/images/UCLH_Jun2022/Nikon /unix/www/html/pbt/wikiData/images/UCLH_Jun2022/CMOS]'''
{|class="wikitable" style="display: inline-table;"
!Collimator Size (mm)
!Monitor Units
!With sheet
!No sheet
|-


Photodiode hexadecimal data: '''/unix/pbt/data/uclh/20220616/DDC232'''
| 5 || 400 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_098.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_098.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_166.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_166.JPG]
</div>
|-


{| class="wikitable"
| 7.5 || 400 || <div class="image150px" style="text-align: center;">
!Run number
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_101.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_101.JPG]
!Beam Energy (MeV)
</div>
!Estimated Range (mm)
|| <div class="image150px" style="text-align: center;">
!Current (nA)
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_163.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_163.JPG]
!Spot size (mm, FWHM)
</div>
!DDC232 FSR (pC)
!DDC232 Integration Time (us)
!Degrader WET (mm)
!Comments
!Photodiode QB Fit
!CMOS QB Fit
!PDL Comparison
!Replay Fit
|-
|-
|0 || Background || N/A || N/A || N/A || 350 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 10 || 400 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_104.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_104.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_160.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_160.JPG]
</div>
|-
|-
|1 || Background || N/A || N/A || N/A || 300 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 12.5 || 400 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_107.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_107.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_157.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_157.JPG]
</div>
|-
|-
|2 || Background || N/A || N/A || N/A || 250 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 15 || 400 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_110.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_110.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_153.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_153.JPG]
</div>
|-
|-
|3 || Background || N/A || N/A || N/A || 200 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 20 || 400 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_113.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_113.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_150.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_150.JPG]
</div>
|-
|-
|4 || Background || N/A || N/A || N/A || 150 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 25 || 300 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_116.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_116.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_147.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_147.JPG]
</div>
|-
|-
|5 || Background || N/A || N/A || N/A || 100 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 30 || 300 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_119.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_119.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_144.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_144.JPG]
</div>
|-
|-
|6 || Background || N/A || N/A || N/A || 50 || 170 || 0 || room light off || N/A || N/A || N/A || N/A
 
| 35 || 250 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_122.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_122.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_141.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_141.JPG]
</div>
|-
|-
|7 || Background || N/A || N/A || N/A || 12.5 || 170 || 0 || room light off (continued)|| N/A || N/A || N/A || N/A
 
| 40 || 250 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_125.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_125.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_138.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_138.JPG]
</div>
|-
|-
|8 || 240 || N/A || 10|| N/A || 350 || 170 || 50 || LH shoot through; 270° gantry; CMOS sensor saturated|| N/A || N/A || N/A || N/A
 
| 50 || 150 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_128.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_128.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_135.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_135.JPG]
</div>
|-
|-
|9 || 240 || N/A || 10 || N/A || 12.5 || 170 || 50 || LH shoot through; 270° gantry; CMOS sensor saturated || N/A || N/A || N/A || N/A
 
| 60 || 150 || <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_095.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_095.JPG]
</div>
|| <div class="image150px" style="text-align: center;">
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_132.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_132.JPG]
</div>
|-
|-
|10 || 240 || N/A || 10 || N/A || 12.5 || 170 || 50 || RH shoot through; 90° gantry; CMOS sensor saturated || N/A || N/A || N/A || N/A
 
|-
|}
|11 || 200 || N/A || 90 || N/A || 12.5 || 170 || 50 || RH shoot through; 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
 
|-
== Before and after irradiation ==
|12 || 200 || N/A || 20 || N/A || 12.5 || 170 || 50 || RH shoot through; 90° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
 
|-
<div class="image300px" style="text-align: center;">
|13 || 130 || N/A || 50 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor slight saturation; || N/A || N/A || N/A || N/A
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/5mm_010.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/5mm_010.JPG]
|-
[https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_092.JPG https://www.hep.ucl.ac.uk/pbt/wikiData/images/Cyberknife_Birmingham_Feb2020/calibration_092.JPG]
|14 || 120 || N/A || 60 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
</div>
|-
Green discolouration observed after irradiation. Only present when the lights are on
|15 || 120 || N/A || 270 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|16 || 110 || N/A || 70 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|17 || 110 || N/A || 300 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|18 || 100 || N/A || 90 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|19 || 100 || N/A || 350 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|20 || 90 || N/A || 110 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
|-
|21 || 90 || N/A || 400 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
|-
|22 || 80 || N/A || 500 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
|-
|23 || 70 || N/A || 700 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
|-
|24 || 130 || N/A || 200 || N/A || 12.5 || 170 || 0 || 90° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|25 || 200 || N/A || 20 || N/A || 12.5 || 170 || 50 || LH shoot through; 270° gantry; CMOS sensor fine; || N/A || N/A || N/A || N/A
|-
|26 || 130 || N/A || 200 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|27 || 120 || N/A || 270 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|28 || 110 || N/A || 300 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|29 || 100 || N/A || 350 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|30 || 90 || N/A || 400 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|31 || 80 || N/A || 500 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|32 || 70 || N/A || 700 || N/A || 12.5 || 170 || 0 || 270° gantry; CMOS sensor saturated; || N/A || N/A || N/A || N/A
|-
|33 || background || N/A || N/A || N/A || 12.5 || 170 || 0 || RevC onwards; 270° gantry || N/A || N/A || N/A || N/A
|-
|34 || 240 || N/A || 13 || N/A || 12.5 || 170 || 50 || LH shoot through; 270° gantry || N/A || N/A || N/A || N/A
|-
|35 || 240 || N/A || 13 || N/A || 12.5 || 170 || 50 || RH shoot through; 90° gantry || N/A || N/A || N/A || N/A
|-
|36 || 110 || N/A || 300 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|37 || 105 || N/A || 350 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|38 || 100 || N/A || 350 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|39 || 95 || N/A || 400 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|40 || 90 || N/A || 400 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|41 || 85 || N/A || 500 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|42 || 80 || N/A || 500 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|43 || 75 || N/A || 700 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A
|-
|44 || 70 || N/A || 700 || N/A || 12.5 || 170 || 0 || 90° gantry; || N/A || N/A || N/A || N/A

Latest revision as of 09:51, 15 September 2022

Second experimental measurements with Cyberknife at Birmingham. Green scintillator thin sheet measurements only.

Setup

Schematic

setup.png

Pre-data Taking Information and Notes

  • Phantom
    • 200mmx200mmx2mm blue scintillator sheet polystyrene base
    • sandwiched by two 200mmx200mmx50mm polystyrene clear plastic blocks
  • 565mm from lens to sheet
  • Check camera in focus with ruler on the sheet
  • Ruler in focus at sheet
  • Scintillator blocks aligned to laser cross hairs
  • 785 mm source to surface distance suitable for output factor/PDD/profile measurements.
  • Wooden box is covered with black cloth
  • Camera height adjusted with wooden plates
  • Top surfaces of plastic blocks and sheet aligned with paper underneath

Data Taking

  • All images taken with
    • Canon EOS 1100D
    • 18-55mm lens
    • f/5
    • 200ISO
    • 30s exposure
  • Beam turned on for a given amount of time within the 30s exposure
  • This is noted in monitor units, with the beam having constant 1000MU/minute
  • Used EOS utility app for controlling camera
  • Image of ruler taken at sheet for pixels to mm conversion
  • 3 images taken at each collimator size with 1 background image each.


Collimator Size (mm) Monitor Units With sheet No sheet
5 400

calibration_098.JPG

calibration_166.JPG

7.5 400

calibration_101.JPG

calibration_163.JPG

10 400

calibration_104.JPG

calibration_160.JPG

12.5 400

calibration_107.JPG

calibration_157.JPG

15 400

calibration_110.JPG

calibration_153.JPG

20 400

calibration_113.JPG

calibration_150.JPG

25 300

calibration_116.JPG

calibration_147.JPG

30 300

calibration_119.JPG

calibration_144.JPG

35 250

calibration_122.JPG

calibration_141.JPG

40 250

calibration_125.JPG

calibration_138.JPG

50 150

calibration_128.JPG

calibration_135.JPG

60 150

calibration_095.JPG

calibration_132.JPG

Before and after irradiation

5mm_010.JPG calibration_092.JPG

Green discolouration observed after irradiation. Only present when the lights are on